Induction of specific immune responses by severe acute respiratory syndrome coronavirus spike DNA vaccine with or without interleukin-2 immunization using different vaccination routes in mice.
Identifieur interne : 003679 ( Main/Exploration ); précédent : 003678; suivant : 003680Induction of specific immune responses by severe acute respiratory syndrome coronavirus spike DNA vaccine with or without interleukin-2 immunization using different vaccination routes in mice.
Auteurs : Hui Hu [République populaire de Chine] ; Xinya Lu ; Ling Tao ; Bingke Bai ; Zhenfeng Zhang ; Yao Chen ; Fangliang Zheng ; Jianjun Chen ; Ze Chen ; Hanzhong WangSource :
- Clinical and vaccine immunology : CVI [ 1556-6811 ] ; 2007.
Descripteurs français
- KwdFr :
- ADN viral (immunologie), Administration par voie orale, Animaux, Anticorps antiviraux (sang), Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Immunoglobuline G (sang), Injections musculaires, Interleukine-2 (génétique), Interleukine-2 (immunologie), Lymphocytes T CD8+ (immunologie), Lymphocytes auxiliaires Th1 (immunologie), Lymphocytes auxiliaires Th2 (immunologie), Modèles animaux, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Salmonella typhimurium (immunologie), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Vaccins antiviraux (immunologie), Vaccins antiviraux (pharmacologie), Vaccins atténués (immunologie), Vaccins atténués (pharmacologie), Virus du SRAS (génétique), Virus du SRAS (immunologie), Électroporation.
- MESH :
- génétique : Glycoprotéines membranaires, Interleukine-2, Protéines de l'enveloppe virale, Virus du SRAS.
- immunologie : ADN viral, Glycoprotéines membranaires, Interleukine-2, Lymphocytes T CD8+, Lymphocytes auxiliaires Th1, Lymphocytes auxiliaires Th2, Protéines de l'enveloppe virale, Salmonella typhimurium, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Vaccins atténués, Virus du SRAS.
- pharmacologie : Vaccins antiviraux, Vaccins atténués.
- sang : Anticorps antiviraux, Immunoglobuline G.
- Administration par voie orale, Animaux, Femelle, Glycoprotéine de spicule des coronavirus, Injections musculaires, Modèles animaux, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère, Électroporation.
English descriptors
- KwdEn :
- Administration, Oral, Animals, Antibodies, Viral (blood), CD8-Positive T-Lymphocytes (immunology), DNA, Viral (immunology), Electroporation, Female, Immunoglobulin G (blood), Injections, Intramuscular, Interleukin-2 (genetics), Interleukin-2 (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Models, Animal, SARS Virus (genetics), SARS Virus (immunology), Salmonella typhimurium (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Th1 Cells (immunology), Th2 Cells (immunology), Vaccines, Attenuated (immunology), Vaccines, Attenuated (pharmacology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (immunology), Viral Vaccines (pharmacology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , genetics : Interleukin-2, Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : SARS Virus.
- immunology : CD8-Positive T-Lymphocytes, DNA, Viral, Interleukin-2, Membrane Glycoproteins, SARS Virus, Salmonella typhimurium, Severe Acute Respiratory Syndrome, Th1 Cells, Th2 Cells, Vaccines, Attenuated, Viral Envelope Proteins, Viral Vaccines.
- chemical , pharmacology : Vaccines, Attenuated, Viral Vaccines.
- prevention & control : Severe Acute Respiratory Syndrome.
- Administration, Oral, Animals, Electroporation, Female, Injections, Intramuscular, Mice, Mice, Inbred BALB C, Models, Animal, Spike Glycoprotein, Coronavirus.
Abstract
DNA vaccines induce humoral and cellular immune responses in animal models and humans. To analyze the immunogenicity of the severe acute respiratory syndrome (SARS) coronavirus (CoV), SARS-CoV, spike DNA vaccine and the immunoregulatory activity of interleukin-2 (IL-2), DNA vaccine plasmids pcDNA-S and pcDNA-IL-2 were constructed and inoculated into BALB/c mice with or without pcDNA-IL-2 by using three different immunization routes (the intramuscular route, electroporation, or the oral route with live attenuated Salmonella enterica serovar Typhimurium). The cellular and humoral immune responses were assessed by enzyme-linked immunosorbent assays, lymphocyte proliferation assays, enzyme-linked immunospot assays, and fluorescence-activated cell sorter analyses. The results showed that specific humoral and cellular immunities could be induced in mice by inoculating them with SARS-CoV spike DNA vaccine alone or by coinoculation with IL-2-expressing plasmids. In addition, the immune response levels in the coinoculation groups were significantly higher than those in groups receiving the spike DNA vaccine alone. The comparison between the three vaccination routes indicated that oral vaccination evoked a vigorous T-cell response and a weak response predominantly with subclass immunoglobulin G2a (IgG2a) antibody. However, intramuscular immunization evoked a vigorous antibody response and a weak T-cell response, and vaccination by electroporation evoked a vigorous response with a predominant subclass IgG1 antibody response and a moderate T-cell response. Our findings show that the spike DNA vaccine has good immunogenicity and can induce specific humoral and cellular immunities in BALB/c mice, while IL-2 plays an immunoadjuvant role and enhances the humoral and cellular immune responses. Different vaccination routes also evoke distinct immune responses. This study provides basic information for the design of DNA vaccines against SARS-CoV.
DOI: 10.1128/CVI.00019-07
PubMed: 17494640
Affiliations:
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type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Lu, Xinya" sort="Lu, Xinya" uniqKey="Lu X" first="Xinya" last="Lu">Xinya Lu</name></author><author><name sortKey="Tao, Ling" sort="Tao, Ling" uniqKey="Tao L" first="Ling" last="Tao">Ling Tao</name></author><author><name sortKey="Bai, Bingke" sort="Bai, Bingke" uniqKey="Bai B" first="Bingke" last="Bai">Bingke Bai</name></author><author><name sortKey="Zhang, Zhenfeng" sort="Zhang, Zhenfeng" uniqKey="Zhang Z" first="Zhenfeng" last="Zhang">Zhenfeng Zhang</name></author><author><name sortKey="Chen, Yao" sort="Chen, Yao" uniqKey="Chen Y" first="Yao" last="Chen">Yao Chen</name></author><author><name sortKey="Zheng, Fangliang" sort="Zheng, Fangliang" uniqKey="Zheng F" first="Fangliang" last="Zheng">Fangliang Zheng</name></author><author><name sortKey="Chen, Jianjun" sort="Chen, Jianjun" uniqKey="Chen J" first="Jianjun" last="Chen">Jianjun Chen</name></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name></author><author><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name></author></analytic><series><title level="j">Clinical and vaccine immunology : CVI</title><idno type="ISSN">1556-6811</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Oral</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>CD8-Positive T-Lymphocytes (immunology)</term><term>DNA, Viral (immunology)</term><term>Electroporation</term><term>Female</term><term>Immunoglobulin G (blood)</term><term>Injections, Intramuscular</term><term>Interleukin-2 (genetics)</term><term>Interleukin-2 (immunology)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Models, Animal</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Salmonella typhimurium (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Spike Glycoprotein, Coronavirus</term><term>Th1 Cells (immunology)</term><term>Th2 Cells (immunology)</term><term>Vaccines, Attenuated (immunology)</term><term>Vaccines, Attenuated (pharmacology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (immunology)</term><term>Viral Vaccines (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (immunologie)</term><term>Administration par voie orale</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Immunoglobuline G (sang)</term><term>Injections musculaires</term><term>Interleukine-2 (génétique)</term><term>Interleukine-2 (immunologie)</term><term>Lymphocytes T CD8+ (immunologie)</term><term>Lymphocytes auxiliaires Th1 (immunologie)</term><term>Lymphocytes auxiliaires Th2 (immunologie)</term><term>Modèles animaux</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Salmonella typhimurium (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins antiviraux (pharmacologie)</term><term>Vaccins atténués (immunologie)</term><term>Vaccins atténués (pharmacologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Électroporation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Interleukin-2</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Interleukine-2</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>ADN viral</term><term>Glycoprotéines membranaires</term><term>Interleukine-2</term><term>Lymphocytes T CD8+</term><term>Lymphocytes auxiliaires Th1</term><term>Lymphocytes auxiliaires Th2</term><term>Protéines de l'enveloppe virale</term><term>Salmonella typhimurium</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Vaccins atténués</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD8-Positive T-Lymphocytes</term><term>DNA, Viral</term><term>Interleukin-2</term><term>Membrane Glycoproteins</term><term>SARS Virus</term><term>Salmonella typhimurium</term><term>Severe Acute Respiratory Syndrome</term><term>Th1 Cells</term><term>Th2 Cells</term><term>Vaccines, Attenuated</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Vaccins antiviraux</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Vaccines, Attenuated</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Administration, Oral</term><term>Animals</term><term>Electroporation</term><term>Female</term><term>Injections, Intramuscular</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Models, Animal</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Administration par voie orale</term><term>Animaux</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Injections musculaires</term><term>Modèles animaux</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère</term><term>Électroporation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">DNA vaccines induce humoral and cellular immune responses in animal models and humans. To analyze the immunogenicity of the severe acute respiratory syndrome (SARS) coronavirus (CoV), SARS-CoV, spike DNA vaccine and the immunoregulatory activity of interleukin-2 (IL-2), DNA vaccine plasmids pcDNA-S and pcDNA-IL-2 were constructed and inoculated into BALB/c mice with or without pcDNA-IL-2 by using three different immunization routes (the intramuscular route, electroporation, or the oral route with live attenuated Salmonella enterica serovar Typhimurium). The cellular and humoral immune responses were assessed by enzyme-linked immunosorbent assays, lymphocyte proliferation assays, enzyme-linked immunospot assays, and fluorescence-activated cell sorter analyses. The results showed that specific humoral and cellular immunities could be induced in mice by inoculating them with SARS-CoV spike DNA vaccine alone or by coinoculation with IL-2-expressing plasmids. In addition, the immune response levels in the coinoculation groups were significantly higher than those in groups receiving the spike DNA vaccine alone. The comparison between the three vaccination routes indicated that oral vaccination evoked a vigorous T-cell response and a weak response predominantly with subclass immunoglobulin G2a (IgG2a) antibody. However, intramuscular immunization evoked a vigorous antibody response and a weak T-cell response, and vaccination by electroporation evoked a vigorous response with a predominant subclass IgG1 antibody response and a moderate T-cell response. Our findings show that the spike DNA vaccine has good immunogenicity and can induce specific humoral and cellular immunities in BALB/c mice, while IL-2 plays an immunoadjuvant role and enhances the humoral and cellular immune responses. Different vaccination routes also evoke distinct immune responses. This study provides basic information for the design of DNA vaccines against SARS-CoV.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement></list><tree><noCountry><name sortKey="Bai, Bingke" sort="Bai, Bingke" uniqKey="Bai B" first="Bingke" last="Bai">Bingke Bai</name><name sortKey="Chen, Jianjun" sort="Chen, Jianjun" uniqKey="Chen J" first="Jianjun" last="Chen">Jianjun Chen</name><name sortKey="Chen, Yao" sort="Chen, Yao" uniqKey="Chen Y" first="Yao" last="Chen">Yao Chen</name><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Lu, Xinya" sort="Lu, Xinya" uniqKey="Lu X" first="Xinya" last="Lu">Xinya Lu</name><name sortKey="Tao, Ling" sort="Tao, Ling" uniqKey="Tao L" first="Ling" last="Tao">Ling Tao</name><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><name sortKey="Zhang, Zhenfeng" sort="Zhang, Zhenfeng" uniqKey="Zhang Z" first="Zhenfeng" last="Zhang">Zhenfeng Zhang</name><name sortKey="Zheng, Fangliang" sort="Zheng, Fangliang" uniqKey="Zheng F" first="Fangliang" last="Zheng">Fangliang Zheng</name></noCountry><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Hu, Hui" sort="Hu, Hui" uniqKey="Hu H" first="Hui" last="Hu">Hui Hu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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